… Streptococcus intermedius is an opportunistic bacterial pathogen secreting a human-specific
cytolysin called intermedilysin … intermedius PC574 was cultured in fetal bovine serum (FBS) …

… Intermedilysin (ILY) is a member of the CDC family produced by Streptococcus intermedius.
ILY is … profile is binding of Δ6eGFPsILY to sheep erythrocytes, included as a negative control. …

… intermedius strains produce a potent human-specific cytoxin, intermedilysin, which shows
little to no activity … Sterile Todd-Hewitt-yeast broth was used as a negative control. Luciferase …

… secretes a human-specific cytolysin intermedilysin (ILY) as a major … Because of the correlation
between higher production of ILY … Because MsgA and NanA activities showed positive and …

… Streptococcus intermedius is a β-hemolytic Gram-positive coccus and member of the
Streptococcus anginosus … intermedilysin and its 3’-flanking region was shown to be specific for S. …

… anginosus group is the intermedilysin, produced by S. … As a negative control, the two
pAT28::sagB vectors were … In contrast to intermedilysin, our experiments demonstrated the …

… It was also found that intermedilysin expression by S. … the fold change in absorbance
relative to the negative control. … intermedius strains, which are known to produce ILY, and the …

… Only intermedilysin from Streptococcus intermedius has … μL erythrocyte suspension for the
positive control of hemolysis (… -inactivated plasma for the negative control of hemolysis (0% …

… related to intermedilysin from Streptococcus intermedius. … The negative control for lysis
consisted of PBS without toxin … and IL-8 pathways in human epithelial cells, suggest that VLY …

… Positive control: ddH 2 O; negative control: assay buffer. The … of alternative sugar utilization
pathways in the presence of … anginosus pBSU409 strain served as negative control 44 …